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Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas ArchiMer
Asmani, Katia; Petton, Bruno; Le Grand, Jacqueline; Mounier, Jerome; Robert, Rene; Nicolas, Jean-louis.
This study has two main objectives: (1) to implement a recycling aquaculture system (RS) for the larvae of the oyster Crassostrea gigas, and (2) to characterise the bacterial communities established in different compartments of this system. An RS with 25% fresh seawater addition per hour and another with no addition (0%) were compared with a flow-through system (FT). Larval survival was equivalent in RS and FT, but growth rate was 17% slower in RS than in FT. The physical chemical parameters remained stable, except for pH that decreased to 7.75 and salinity that increased to 37.5‰ in the RS 0%. In both systems, the cultivable bacteria were present in similar numbers in seawater (around 105 ml− 1) and in larvae (103 larva− 1) on day 15. Bacterial...
Tipo: Text Palavras-chave: RAS; Bivalve larvae; Microbiota; Pyrosequencing; Hatchery.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00346/45691/45311.pdf
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The influence of techniques of larvae rearing and seed collectors on the survival rate and recovery efficiency of the brown mussel Perna perna (L.) in laboratory BABT
Beduschi,Pedro; Melo,Cláudio Manoel Rodrigues de; Ferreira,Jaime Fernando.
Te study compared the hatchery recovery efficiency and survival rate of Perna perna spats using larvae submitted to four conditions, prior to settlement, tested with for different collectors. In 15 days old larvae (eyed larvae, T1), transparent nylon thread collectors presented the best results for spats recovered per meter of collector. The 28 days old larvae (foot larvae) stored under refrigeration before settlement (T3 with water and T4 without water) showed no significant differences between the spat number in all the collectors. The brown multi-thread collector was more efficient in T 4. The blue polyamide thread collector was the most efficient with 28 days old larvae settled directly, without refrigeration (T2). Treatment 1 showed the highest spats...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spats; Settlement; Perna perna; Collectors; Hatchery.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100019
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Etude des processus de dérive et de sélection liés aux pratiques d'élevage en écloserie d'huître creuse ArchiMer
Boudry, Pierre.
Genetic consequences of production of Pacific oyster larval in hatchery: drift and selective pressures related to rearing practices. In order to study the genetic consequences of production of oyster larvae in hatcheries, two factors were examined: the effects of discarding the smallest larvae (i.e. culling) and temperature effects. A mixed-family approach was used in order to infer the genetic composition of the larval population. The results show that high polymorphic microsatellite-based family assignment is a powerful tool for the study of bivalve larvae genetics. Culling by selective sieving is an advantageous practice at a phenotypic scale, but also represents a substantial risk for diversity loss if parentage assignment is not introduced as a...
Tipo: Text Palavras-chave: Hatchery; Genetic diversity; Selection domestication; Larvae; Crassostrea gigas; Ecloserie; Diversité génétique; Sélection domestication; Larve; Crassostrea gigas.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/rapport-1459.pdf
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Microsatellite markers as a tool to study reproductive success in the Pacific oyster, Crassostrea gigas (Thunberg), under controlled hatchery conditions ArchiMer
Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Bonhomme, Francois.
Oysters, like many marine species have a very high fecundity. Previous studies have shown that populations, from both hatcheries and the natural environment, have very low Ne/N ratios. These observations reveal high variation in reproductive success. In order to study individual reproductive success under controlled conditions, we used microsatellite markers to quantify parental contributions in in vitro crosses (5 males and 5 females) of Crassostrea gigas, the Pacific oyster. High polymorphism of the microsatellites (more that 50 alleles per locus) eased the parentage identifications. The results of a cross allowing gametic competition were compared with the results from a second cross where the gametes of the same parents were kept separate for each...
Tipo: Text Palavras-chave: Genetic; Hatchery; Microsatellite markers; Crassostrea gigas; Pacific oysters.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/acte-3457.pdf
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Le système immunitaire du bar Dicentrarchus labrax. Immunoglobulines, cellules productrices d'immunoglobulines, ontogenèse applications à une pathologie virale néonatale ArchiMer
Breuil, Gilles.
The sea bass, Dicentrarchus labrax, has been subjected to intensive aquaculture on the Mediterranean cost for ten years. Marine fish hatcheries are key factors in the aquaculture field, and face different pathologies, especially one of viral origin (nodavirus) that could challenge their feasibility. Consequently, we have tried to provide some data on larvae and spawning stocks. Firstly, we have studied the immunoglobulins, components of the specific humoral immunity, and their role as antibodies. On adult basses, these immunoglobulins are tetrameric molecules with high molecular weight (850 kD), made of heavy (H) - 80 kD and light (L) - 26 kD chains. In the eggs, simplest forms such as monomeric or dimeric are brought to the fore; they are located under...
Tipo: Text Palavras-chave: Egg; Cytometry; Immunity system; Larvae; Immunoglobulin; Nodavirus; Dicentrarchus labrax; Hatchery; Ontogenese; Cytometrie; Immunoglobuline; Nodavirus; Oeuf; Larve; Nurserie; Dicentrarchus labrax.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/these-1265.pdf
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Production artificielle de naissain de coquilles Saint-Jacques Pecten maximus (L.). Premiers résultats en rade de Brest ArchiMer
Buestel, Dominique; Cochard, Jean-claude; Dao, Jean-claude; Gerard, Andre.
Artificial production from natural spawners could be a way of getting big quantities of spat of Pecten maximus, ln the method described, larval phase (until a size of 250 microns) occurs in a hatchery, post-Iarval phase (from 250 µ until 3 millimeters) in a nursery, and first growth period directly into the sea (from 2 mm to a size of 25-30 mm), The fifst results obtained, production of several thousand of individuals good enough for reseeding, seem to be transposable to a larger scale.
Tipo: Text Palavras-chave: Restocking; Intermediate culture; Hatchery; Nursery; Pecten maximus; Repeuplement; Prégrossissement; Ecloserie; Nurserie; Pecten maximus.
Ano: 1982 URL: http://archimer.ifremer.fr/doc/1982/publication-6529.pdf
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Optimization of heterologous microsatellites in Piracanjuba PAB
Carmo,Fausto Moreira da Silva; Polo,Érico Macedo; Silva,Marcos Antônio da; Yazbeck,Gabriel de Menezes.
Abstract: The objective of this work was to evaluate 41 microsatellite markers for heterologous amplifications in piracanjuba (Brycon orbignyanus). Some markers were tested for the first time. Loci were optimized for PCR conditions and applied to a sample of 49 individuals. Thirty-one loci resulted in PCR product formation, whereas ten loci yielded intelligible polymorphic patterns in the evaluated sample and can be used for amplifications in this species. From the evaluated markers, four loci (BoM1, BoM13, Bh6, and Bh16) are valid to be applied in the study of piracanjuba.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brycon orbignyanus; Aquaculture; Hatchery; Molecular markers.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2015001201236
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Action of Antimicrobial Copper on Bacteria and Fungi Isolated from Commercial Poultry Hatcheries Rev. Bras. Ciênc. Avic.
Depner,RFR; Pontin,KP; Depner,RA; Flores Neto,A; Lucca,V; Lovato,M.
ABSTRACT Since 2008, when the US Environmental Protection Agency (EPA) registered copper and its alloys as an antimicrobial agent for contact surfaces, research has demonstrated their antimicrobial activity. The aim of this study was to evaluate the efficacy of antimicrobial copper against bacteria and fungi isolated from commercial poultry hatcheries in order to develop a microbiological control alternative in these environments. Samples were collected from the surfaces of hatcher baskets from two hatcheries. Mesophilic microorganisms and fungi/yeasts were isolated and standardized in concentration of 105 cells/mL. Four copper plates and four stainless steel plates were completely immersed for one minute in bacteria and fungi/yeasts solutions and left to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial copper; Hatchery; Stainless steel; Copper surfaces.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000600095
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Review of the reproductive physiology of the scallop, Pecten maximus, applicable to intensive aquaculture ArchiMer
Devauchelle, Nicole; Mingant, Christian.
This paper presents a summary of information on reproduction biology in the scallop, Pecten maximus, which is required for the successful reseeding and intensive aquaculture programs developed in France since 1982. Data are presented on the following subjects: environmental factors that sustain gametogenic activity and the time of year this activity occurs; biochemical changes that are associated with gametogenesis; the consistency of egg quality; and development of a simple test to predict egg quality.
Tipo: Text Palavras-chave: Aquaculture; Pecten maximus; Ecloserie; Physiologie de la reproduction; Embryon; Gamète; Aquaculture; Pecten maximus; Scallop; Hatchery; Reproductive physiology; Egg quality; Embryo; Gamete.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/00038/14890/12216.pdf
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1983-2002 : The 20-year development of the King scallop (Pecten maximus) sea-farming industry in the bay of Brest (France) :historical record, results, prospect ArchiMer
Fleury, Pierre-gildas; Carval, Jean-pierre; Muzellec, Marie-louise; Gerard, Andre; Barret, Jean; Cochard, Jean-claude; Dao, Jean-claude.
Tipo: Text Palavras-chave: France; Fishing management; Seeding; Hatchery; Pecten maximus; King scallop.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-7307.pdf
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L'élevage larvaire de crevettes en Nouvelle-Calédonie. L'Oxytétracycline et l'association Triméthoprime - Sulfadiazine en tant qu'alternatives à l'emploi de l'Erythromycine en écloserie ArchiMer
Francois, Cyrille.
Aquaculture of penaeid shrimps holds a major place in productions of animals. New Caledonia takes part to these productions by developing the breeding of Pacific blue shrimp Litopenaeus stylirostris. Nowadays, penaeid larviculture can’t be undertaken unless antibiotics are used. Experiments in the Caledonian Aquacultural Laboratory (LAC IFREMER Saint-Vincent) put across to the development of two antibiotic prophylaxis plans for substitution of erythromycine, one plan with oxytetracycline (hydrochloride), the other plan with trimethoprime-sulfadiazine association. After an antimicrobial agent susceptibility testing of bacteria and an acute toxicity study, efficacity assays are the biological validation of these projects of protocols
Tipo: Text Palavras-chave: Crevette; Ecloserie; Oxytétracycline; Triméthoprime; Sulfadiazine; Shrimp; Hatchery; Oxytetracycline; Trimetroprime; Sulfadiazine.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00072/18312/15877.pdf
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Analysis of hemocyte parameters in Pacific oysters, Crassostrea gigas, reared in the field — Comparison of hatchery diploids and diploids from natural beds ArchiMer
Gagnaire, Beatrice; Soletchnik, Patrick; Faury, Nicole; Kerdudou, Nolwenn; Le Moine, Olivier; Renault, Tristan.
Internal defence mechanisms of bivalves include the hemocytes which assume several functions. Relationships exist between environmental factors, physiological state of oysters, defence system and pathogens. Hatchery oysters are more and more produced and reared in the field. The characteristics and particularly the defence system of these animals are poorly documented. In this context, two field trials were carried out from May to September 2002 and from April to July 2003 in order to monitor hemocyte parameters of diploid oysters from natural beds and hatchery-produced diploid oysters using flow cytometry. Several hemocyte parameters (cell mortality, granulocyte percentage, phagocytosis, esterase and peroxidase activities) were monitored. For both oyster...
Tipo: Text Palavras-chave: Hatchery; Natural beds; Diploids; Flow cytometry; Peroxidase activity; Esterase activity; Phagocytosis; Hemocytes; Crassostrea gigas; Pacific oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3119.pdf
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Effect of the probiotic strain Phaeobacter gallaeciensis after bacterial challenge on the complete larval development of Pecten maximus ArchiMer
Genard, Bertrand; Larouche, Olivier; Nicolas, Jean-louis; Miner, Philippe; Beaudin, Marie-lou; Tremblay, Rejean.
The aim of this project was to evaluate the impact of probiotic (Phaeobacter gallaeciensis, X34 strain) treatment on the complete development (from veliger to metamorphosis) of Pecten maximus larvae in the context of a bacterial challenge and in conditions more representative of hatchery practices. To that effect, the present study was divided into two main steps. In the first, we used in vitro analyses (antibiograms and microplate assays) to validate the inhibition abilities of X34 on the growth of four Vibrio pathogen species. During the second step, we added pathogens (Vibrio pectenicida) into rearing tanks after two weeks of pre-treatment with the probiotic and then followed the larval development of Pecten maximus through the monitoring of survival...
Tipo: Text Palavras-chave: Probiotics; Hatchery; Bacterial challenge; Larval development; Pecten maximus; Phaeobacter gallaeciensis; Vibrio.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00213/32429/30895.pdf
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Etude comparative du cycle de reproduction de la palourde Ruditapes decussatus en milieu naturel (sud Tunisie) et contrôlé (écloserie) ArchiMer
Hamida, Leila; Medhiouband, Mohamed; Cochard, Jean-claude; Romdhane, Mohamed; Le Pennec, Marcel.
A comparative study of the reproductive cycle of Ruditapes decussatus under natural (South of Tunisia) and controlled conditions (hatchery). The aim of this study was to describe and compare the gametogenic activity of Ruditapes decussatus in situ and in hatchery. Criteria of maturity were characterized and the positive role of conditioning in the success of artificial reproduction was underlined. Specimens sampled in the South of Tunisia were conditioned at the experimental station of Monastir (Tunisia). The parameters studied were the variation of the condition index and the frequency distribution of oocyte diameters. Results showed that the reproductive cycle was continuous in situ. Gametogenesis started in January and February. Gonadic activity...
Tipo: Text Palavras-chave: Ruditapes; Natural conditions; Hatchery; Reproduction; Gonatic activity; Gametogenesis.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-882.pdf
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La sensibilité des larves de pectinidés aux conditions d’élevage : le flux ouvert comme alternative aux mortalités massives ArchiMer
Holbach, Marine.
In many countries, aquaculture of pectinids depends on the success of artificial spat production in hatchery. This production is always unpredictable due to the variability of hatching rate and larval survival. Flow-through larval rearing systems were developed in Norway for the King scallop Pecten maximus and showed promising results. Unfortunately the system needs to be optimized since the larval yields and the densities used are still relatively low. In France, a small-scale (5 L) and high-density (≤ 300 larva mL-1) flow-through larval rearing system was successfully developed for oysters. First trials in such system and in similar conditions with P. maximus failed as we registered slower growth and high mortality rate in only a few days. It is known...
Tipo: Text Palavras-chave: Pectinidés; Pecten maximus; Écloserie; Élevage larvaire; Flux ouvert; Contamination bactérienne; Prophylaxie; Physiologie larvaire; Indices de stress.; Pectinids; Pecten maximus; Hatchery; Larval rearing; Flow-through; Bacterial contamination; Prophylaxis; Larval physiology; Stress index.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00259/37000/35491.pdf
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Robustness or resilience? Managing the intersection of ecology and engineering in an urban Alaskan fishery Ecology and Society
Krupa, Meagan B.; Alaska Pacific University; mkrupa@alaskapacific.edu; Chapin, III, F. Stuart; University of Alaska Fairbanks ; terry.chapin@alaska.edu; Lovecraft, Amy L.; University of Alaska Fairbanks; allovecraft@alaska.edu.
Tipo: Peer-Reviewed Reports Palavras-chave: Hatchery; Robustness theory; Salmon; Social-ecological systems; Stream management; Urban fishery.
Ano: 2014
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Cryopreservation of Pacific oyster ( Crassostrea gigas ) larvae: Revisiting the practical limitations and scaling up the procedure for application to hatchery ArchiMer
Labbe, Catherine; Haffray, Pierrick; Mingant, Christian; Quittet, Benjamin; Diss, Blandine; Tervit, H. Robin; Adams, Serean L.; Rimond, Flore; Suquet, Marc.
Pacific oyster Crassostrea gigas is one major species for aquaculture, and the development of breeding programs and the need for preservation of wild stock genetic resources prompted the need for larvae cryopreservation. The objective of the present study was to choose the most reliable protocol from several existing publications, to test its biological and practical limitations, and to adapt it to hatchery conditions. The selected protocol was characterized by a very slow freezing rate without seeding, and by the use of ethylene glycol and sucrose as cryoprotectant. The best survivals after thawing and rearing up to 48 h post fertilization (hpf) were obtained with larvae that were frozen at late trochophore (20 hpf) and early-D (24 hpf) stages. Increasing...
Tipo: Text Palavras-chave: Oyster; Cryobanking; D stage; Ethylene glycol; High throughput; Hatchery.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00423/53460/54354.pdf
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Cultures et élevages de masse dans le contexte d'une écloserie-nurserie de mollusques bivalves : les productions phytoplanctoniques et le grossissement des post-larves ArchiMer
Le Borgne, Y.; Marin, J.; Vergonzanne, G..
The hatchery-nursery from the Société Atlantique de Mariculture settled in Barfleur studied within the ECOTRON program set up by C.N.E.X.O. the characteristics of mass cultures for monospecific phytoplancton and of the rearing of oyster spat (Ostrea edulis. Crassostrea giqas) and clam spat (Venerupis semi­decussata) . The maintenance features of the cultures in order to obtain cell densities around 1 million cell/ml. (or 50 g. of dry weight/m3) in containers up to 5000 liters have been established for the main species used in aquaculture (Isochrysis qalbana. Monochrysis lutheri, Tetraselmis suecica. Skeletonema costatum. Phaeodactylum tricornutum. Cyclotella nana, Chaetoceros calcitrans). The spat growth in nursery without providing any cultured food...
Tipo: Text Palavras-chave: Écloserie; Nurserie; Phytoplancton; Bivalves; Naissain; Hatchery; Nursery; Phytoplancton; Bivalves; Spat.
Ano: 1979 URL: http://archimer.ifremer.fr/doc/00305/41658/40867.pdf
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Parallel epigenetic modifications induced by hatchery rearing in a Pacific Salmon ArchiMer
Le Luyer, Jeremy; Laporte, Martin; Beacham, Terry D.; Kaukinen, Karia H.; Withler, Ruth E.; Leong, Jong S.; Rondeau, Eric B.; Koop, Ben F.; Bernatchez, Louis.
A puzzling question in conservation biology is how to maintain overall fitness of individuals  bred in captive environment upon release into the wild, especially for rehabilitating declining or threatened species [1,2]. For salmonid species, a heritable change in fitness related traits and gene expression has been reported to occur in a single generation of captivity in hatchery environment [3–5]. Such rapid changes are congruent with models of  inadvertent domestication selection which may lead to maladaptation in the natural environment [4]. Arguably, the underlying mechanism by which captivity may induce  fitness difference between wild and captive congeners is still poorly understood. Short- term selection on complex phenotypic traits is expected to...
Tipo: Text Palavras-chave: Epigenetics; Methylation; Coho salmon; Hatchery; RAD sequencing.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00409/52050/52722.pdf
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Epigenomic modifications induced by hatchery rearing persist in germ line cells of adult salmon after their oceanic migration ArchiMer
Leitwein, Maeva; Laporte, Martin; Le Luyer, Jeremy; Mohns, Kayla; Normandeau, Eric; Withler, Ruth; Bernatchez, Louis.
Human activities induce direct or indirect selection pressure on natural population and may ultimately affect population’s integrity. While numerous conservation programs aimed to minimize human‐induced genomic variation, human‐induced environmental variation may generate epigenomic variation potentially affecting fitness through phenotypic modifications. Major questions remain pertaining to how much epigenomic variation arises from environmental heterogeneity, whether this variation can persist throughout life, and whether it can be transmitted across generations. We performed whole genome bisulfite sequencing (WGBS) on the sperm of genetically indistinguishable hatchery and wild born migrating adults of Coho salmon (Oncorhynchus kisutch) from two...
Tipo: Text Palavras-chave: Conservation; Developmental plasticity; Epigenomic; Fisheries; Fitness; Hatchery; Salmonid.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00688/79977/82923.pdf
Registros recuperados: 42
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